We hypothesise that patients with SARS-Cov-2 infection are characterized by progressivechanges in distribution of distinct lung macrophages populations mediated by influx ofcirculating monocytes into the lungs . Moreover, we also hypothesise that patients withhigher rate of MerTKpos alveolar macrophages in the lung lavage will have the lowest rateof lung complications and the best recovery outcome in terms of clinical outcome and needof assisted ventilation supporting the use of macrophage phenotyping as novel prognosticbiomarker in patients with SARS-Cov-2 infection. Finally, the definition of thetranscriptomic signature of peripheral blood and tissue-derived myeloid cell subtypeswill offer new therapeutic target of this uncurable newly discovered infection.
Not Provided
Other: Immune-phenotyping of tissue and peripheral blood myeloid compartment
Peripheral blood sampling and bronchoalveolar lavage fluid collection based on the
clinical indication at baseline (T0) and peripheral blood sample at the time of clinical
worsening/improvement during the follow-up (T1). Moreover, after complete recovery and
discharge from the hospital peripheral blood sampling in the outpatient clinical
assessment will be performed (T2).
BALF and peripheral blood will be processed for immune-phenotyping of the myeloid
compartment using single-cell RAN sequencing analysis.
Inclusion Criteria:
- Patients with COVID-19 pneumonia
Exclusion Criteria:
- Other infections rather than Covid-19
- Patients with concomitant treatment with prednisone ≥10 mg daily
- Patients under therapy with immunosuppressants
Division of Rheumatology
Roma, Lazio, Italy
School of Infection and Immunity
Glasgow, United Kingdom
Not Provided